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Shifts of archaeal community structure in soil along an elevation gradient in a reservoir water level fluctuation zone
Ye, Fei1,2; Wu, Shengjun1; Jiang, Yi1; Op den Camp, Huub J. M.3; Li, Zhe1; Zhu, Guibing4; Zheng, Jun5; Wang, Yu1
2016-12-01
摘要Purpose Although archaea play an important role in nutrients cycling, the archaeal community in a reservoir water-level fluctuation zone (WLFZ) remains unclear. An elucidation of archaeal community responding to the environmental variables is essential to understand the nutrients dynamics in WLFZ. This study focused on the response of the archaeal community structure and abundance to the periodic water flooding along an elevation gradient in the WLFZ of the Three Gorges Reservoir. Materials and methods Along the elevation gradient (152-175 m) of the study area, soil samples in the beginning and late stages of water flooding were collected to investigate the influence of water flooding on the archaeal community in soil, using quantitative PCR and Illumina high-throughput sequencing approaches. Results and discussion An increase of archaeal abundance from 3.8 x 10(8) to 3.8 x 10(9) copies (g d.w.s)(-1) on average was observed after water flooding. The archaeal abundance was positively correlated with the contents of ammonium, organic matter, and moisture in soil and with the accumulated flooding time. Higher diversity was observed in dry samples (non-flooded soil samples) rather than wet samples (flooded soil samples). The Thaumarchaeota were predominant in most of the dry samples. Interestingly, high proportions of Candidatus Nitrososphaera were observed in the transition zone, while euryarchaeotal methanogens dominated the wet samples. The proportion of methanogens decreased dramatically in the dry samples at higher elevations, which was associated with the decrease of the moisture content and the probably increase of available oxygen in soil. Conclusions Archaeal abundance, diversity, and community composition shifted along an elevation gradient and were influenced by water flooding. The increased archaea abundance after water flooding and elevation related community composition and diversity indicated that water flooding was a key dynamic environmental variable in the WLFZ.
关键词Archaeal community High-throughput sequencing Quantitative PCR Three Gorges Reservoir Water flooding Water level fluctuation zone
DOI10.1007/s11368-016-1485-3
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发表期刊JOURNAL OF SOILS AND SEDIMENTS
ISSN1439-0108
卷号16期号:12页码:2728-2739
通讯作者Wang, Y (reprint author), Chinese Acad Sci, Chongqing Inst Green & Intelligent Technol, Chongqing 400714, Peoples R China.
收录类别SCI
WOS记录号WOS:000387316000009
语种英语