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	Probe-assisted detection of Fe3+ions in a multi-functionalized nanopore
	Arora, Pearl1; Zheng, Haiyan2; Munusamy, Sathishkumar2; Jahani, Rana2; Wang, Liang3,4; Guan, Xiyun2
	2024-05-01
摘要	Iron is an essential element that plays critical roles in many biological/metabolic processes, ranging from oxygen transport, mitochondrial respiration, to host defense and cell signaling. Maintaining an appropriate iron level in the body is vital to the human health. Iron deficiency or overload can cause life -threatening conditions. Thus, developing a new, rapid, cost-effective, and easy to use method for iron detection is significant not only for environmental monitoring but also for disease prevention. In this study, we report an innovative Fe3+ detection strategy by using both a ligand probe and an engineered nanopore with two binding sites. In our design, one binding site of the nanopore has a strong interaction with the ligand probe, while the other is more selective toward interfering species. Based on the difference in the number of ligand DTPMPA events in the absence and presence of ferric ions, micromolar concentrations of Fe3+ could be detected within minutes. Our method is selective: micromolar concentrations of Mg2+, Ca2+, Cd2+, Zn2+, Ni2+, Co2+, Mn2+, and Cu2+ would not interfere with the detection of ferric ions. Furthermore, Cu2+, Ni2+, Co2+, Zn2+, and Mn2+ produced current blockage events with quite different signatures from each other, enabling their simultaneous detection. In addition, simulated water and serum samples were successfully analyzed. The nanopore sensing strategy developed in this work should find useful application in the development of stochastic sensors for other substances, especially in situations where multi-analyte concurrent detection is desired.
关键词	Multi-functionalized nanopore Molecular probe Ferric ions Multi-analyte simultaneous detection Single -molecule sensor
DOI	10.1016/j.bios.2024.116125
发表期刊	BIOSENSORS & BIOELECTRONICS
ISSN	0956-5663
卷号	251页码:7
通讯作者	Wang, Liang(wangliang83@cigit.ac.cn) ; Guan, Xiyun(xgpc2@missouri.edu)
收录类别	SCI
WOS记录号	WOS:001185593600001
语种	en