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	Role of Capsular Polysaccharides in Biofilm Formation: An AFM Nanomechanics Study
	Wang, Huabin1,3; Wilksch, Jonathan J.2; Strugnell, Richard A.2; Gee, Michelle L.1
	2015-06-17
摘要	Bacteria form biofilms to facilitate colonization of biotic and abiotic surfaces, and biofilm formation on indwelling medical devices is a common cause of hospital-acquired infection. Although it is well-recognized that the exopolysaccharide capsule is one of the key bacterial components for biofilm formation, the underlying biophysical mechanism is poorly understood. In the present study, nanomechanical measurements of wild type and specific mutants of the pathogen, Klebsiella pneumoniae, were performed in situ using atomic force microscopy (AFM). Theoretical modeling of the mechanical data and static microtiter plate biofilm assays show that the organization of the capsule can influence bacterial adhesion, and thereby biofilm formation. The capsular organization is affected by the presence of type 3 fimbriae. Understanding the biophysical mechanisms for the impact of the structural organization of the bacterial polysaccharide capsule on biofilm formation will aid the development of strategies to prevent biofilm formation.
关键词	bacteria capsule polysaccharides fimbriae biofilm nanomechanics
DOI	10.1021/acsami.5b03041
发表期刊	ACS APPLIED MATERIALS & INTERFACES
ISSN	1944-8244
卷号	7期号:23页码:13007-13013
通讯作者	Strugnell, RA (reprint author), Univ Melbourne, Dept Microbiol & Immunol, Parkville, Vic 3010, Australia.
收录类别	SCI
WOS记录号	WOS:000356753500080
语种	英语