中国科学院重庆绿色智能技术研究院机构知识库

QQ客服 官方微博 反馈留言

	Detection of carcinoembryonic antigens using a wavy gold-silver alloy nanoplate enhanced surface plasmon resonance imaging biosensor
	Wu, Jiangling1; Zhang, Qiongyuan1; Kang, Lina1; Wu, Xiaotian1; Li, Daikun1; Wang, Yi3; Huang, Yu2; Xue, Jianjiang1
	2022-11-24
摘要	Carcinoembryonic antigen (CEA) is regarded as a promising broad spectrum tumor biomarker for clinical diagnosis, progression, and prognosis. Surface plasmon resonance imaging (SPRi) was considered as one of the powerful tools for immunoassay with advantages of label-free, real-time detection with high-throughput. Herein, wavy gold-silver alloy nanoplates functionalized with anti-CEA antibodies providing high protein loading capacity and high mass are used as signal enhancers for CEA detection through SPRi sandwich assay. The present method exhibits a dynamic range for CEA determination from 0.1 to 312.5 ng mL(-1) and a detection limit of 0.55 ng mL(-1), well below normal physiological levels. This biosensing approach demonstrates the advantages of wavy gold-silver alloy nanoplates compared to conventional gold nanoparticles as a signal amplifier to enhance the SPRi signal, which is expected to become a new prospect for detection of cancer markers in biomedical research and clinical diagnosis.
DOI	10.1039/d2ay01523d
发表期刊	ANALYTICAL METHODS
ISSN	1759-9660
卷号	14期号:45页码:4713-4720
通讯作者	Huang, Yu(huangyu@cigit.ac.cn) ; Xue, Jianjiang(jianjiangxue@126.com)
收录类别	SCI
WOS记录号	WOS:000890142400001
语种	英语