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Bacterially self-assembled encapsulin nanocompartment for removing silver from water
Xing, Chong-Yang1,2; Ma, Teng-Fei4; Guo, Jin-Song3; Shen, Yu4; Yan, Peng3; Fang, Fang3; Chen, You-Peng1,2,3
2021-03-01
摘要Compartmentalization can protect cells from the interference of external toxic substances by sequestering toxic products. We hypothesized that proteinaceous nanocompartments may be a feasible candidate material to be added to genetically modified bacteria for the sequestration of toxic environmental products, which would open up a new bioremediation pathway. Here, we showed that the model bacterium (Escherichia coli) with self-assembling nanocompartments can remove silver (Ag) from water. Transmission electron microscopy and energy dispersive X-ray (TEM-EDX) analysis showed that the nanocompartments combined stably with silver in vitro . In addition, when exposed to 30 mu M AgNO3, the survival rate of genetically modified bacteria (with nanocompartments) was 86%, while it was just 59% in the wild-type bacteria (without nanocompartments). Label-free quantitative proteomics indicated that the nanocompartments enhanced bacterial activity by inducing the up-regulation of protein processing and secondary metabolites, and decreased their intracellular silver concentration, both of which contributed to their increased resistance to toxic silver. This study on nanocompartments has contributed to a deeper understanding of how bacteria respond to environmental stressors like heavy metal pollutants in water. The technology promises to provide a new strategy for recycling heavy metals from sewage. (c) 2020 Elsevier Ltd. All rights reserved.
关键词Protein cage Self-assembly Bioremediation Silver Proteomics
DOI10.1016/j.watres.2020.116800
发表期刊WATER RESEARCH
ISSN0043-1354
卷号191页码:10
通讯作者Chen, You-Peng(ypchen@cqu.edu.cn)
收录类别SCI
WOS记录号WOS:000615940000005
语种英语