中文版 | English
CSpace
Reduction chemistry-assisted nanopore determination method for immunoglobulin isotypes
Liu, Qianshan1,2; Wang, Yunjiao1,2; Liu, Yaqing1; Wang, Han1,2; Li, Wei1,2; Tang, Peng1; Weng, Tin1; Zhou, Shuo1; Liang, Liyuan1,2; Yuan, Jiahu1,2
2020-10-14
摘要Immunoglobulins can bind to an unlimited array of foreign antigens presented to the immune system. Among those isotypes, IgG and IgM play crucial roles in initial immune defense associated with innate immunity factors. Hence, the determination of IgG and IgM deficiencies or varying concentrations is widely used as a diagnostic indicator for immune deficiency disorders. Herein, we report a reduction chemistry-assisted nanopore method for IgG and IgM determination. TCEP (tris(2-carboxyethyl)phosphine) was used to cleave Ig proteins in fragments by means of disulfide bond reduction under different experimental conditions. This strategy enabled the observation of distinguishable current signals afforded by separated polypeptide fragments in an alpha HL nanopore. Together with molecular dynamics (MD) simulation results, highly effective electrostatic potentials and H-bonds, the dominant factors for these current signals, facilitated the capture of Ig fragments in an alpha-HL nanopore. More importantly, the signature signals were applicable for differentiating between IgG and IgM in blood serum without any problems of protein adsorption and clogging in the nanopore sensing. Furthermore, with comparative sensing sensitivity and selectivity, it is concluded that our method is a label-free single-molecule approach to measuring disease states that present as a result of the absence or over presence of immunoglobulin isotypes.
DOI10.1039/d0nr04900j
发表期刊NANOSCALE
ISSN2040-3364
卷号12期号:38页码:19711-19718
通讯作者Yuan, Jiahu() ; Wang, Deqiang(dqwang@cigit.ac.cn) ; Wang, Lang(yjh@cigit.ac.cn)
收录类别SCI
WOS记录号WOS:000578100000016
语种英语